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. 2010 Aug 3;299(4):E640–E646. doi: 10.1152/ajpendo.00063.2010

Fig. 3.

Fig. 3.

Effects of estrogen treatment on SK3 channel expression in uteri of ovariectomized mice. Uterine whole cell lysates were prepared 3 wk after ovariectomy and pellet implantation (17β-estradiol or placebo), and proteins were separated by SDS-PAGE. Blots were probed with antibodies against SK3 and GAPDH (loading control). A: SK3 protein decreased in the presence of estrogen but remained constant with placebo treatment. B: quantification of SK3 protein levels normalized to GAPDH loading control (n = 6). Means ± SE. *P < 0.05 vs. placebo. C: immunohistochemistry of the uterus 3 wk post-placebo or -estradiol treatment, with localization of SK3 channel expression shown in green and α-smooth muscle actin (SMA) in red.