Figure 1.
IL-12 induces anti-tumor activities in vivo. (a) 4T1 tumor cells were injected s.c. in the abdominal mammary gland with 0.1 ml of a single-cell suspension containing 105 tumor cells. Tumor growth was monitored every 2–3 days and tumor size in diameter (mm) was measured with an electronic caliper. Each data point is comprised of 10 mice. Error bars represent standard deviation. IL-12 treatment started on Day 7 and was injected i.p. every 2 days until day 21. (b) Lung metastasis was measured at the end of this experiment (day 23) by the 6-thiogaunine clonogenicity assay. The dashed horizontal lines represent the mean of each group. (c,d) The same data shown in (a,b) are plotted on X and Y axis to show the correlation between the size of the primary tumors and their ability to metastasize to the lung.