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. 2010 May 10;118(10):1363–1369. doi: 10.1289/ehp.0901661

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ROS levels after exposure of neuronal networks to different concentrations of CB, Fe₂O₃, or TiO₂ NPs for 24 hr. (A) Cell autofluorescence (“Blank”) and the changes in fluorescence intensity and peak distribution of rhodamine 123 analyzed by flow cytometry (DHR assay), in control cells and in cells exposed to NPs. (B) ROS formation in neuronal cells after exposure to different concentrations (0.5–10 μg/cm²) of NPs. Values shown are the mean ± SD of experiment/control (E/C) data from three independent experiments (and preparations) performed in triplicate.

^#p < 0.001.