Table 3.

Calculated Free Nucleotide Concentrations and Ratios

	Saline	Ethanol	Acetate
Free [NAD+]/[NADH] cytosol from lactate/pyruvate	392.7 ± 57.3	397.2 ± 36.9	257.3 ± 33.5b
Free [NAD+]/[NADH] mitochondria from αKG × NH4+/Glut	1.03 ± 0.05	1.04 ± 0.05	1.32 ± 0.08a,b
Free [NADP+]/[NADPH] cytosol from αKG × CO2/IsoCit	0.0130 ± 0.0003	0.0133 ± 0.0010	0.0165 ± 0.0014
Free mitochondrial [CoQ]/[CoQH2] from measured Fum/Succ	0.0047 ± 0.0004	0.0056 ± 0.0006	0.0062 ± 0.0004a
Free [Mg2+] mM from [Cit]/[Isocit]	0.333 ± 0.129	0.261 ± 0.068	0.750 ± 0.181b
Phosphorylation potential from kg + g (Eq. 1)/M	39,315 ± 5,379	43,807 ± 7,528	33,082 ± 3,992
ΔG′ ATP kJ/mol	−61.4 ± 0.4	−61.3 ± 0.5	−59.9 ± 0.3a,b
Free [ADP] cytosol μM/g from PCr/Cr	0.029 ± 0.006	0.043 ± 0.016	0.035 ± 0.006
Free [AMP] cytosol μM/g from Kmyokinase	0.0003 ± 0.00008	0.0009 ± 0.0006	0.0006 ± 0.0001

Animals infused with solutions of phosphate buffered saline (PBS) (n = 5), 4 M ethanol in PBS (n = 8), or 2 M sodium acetate in PBS (n = 6). Values are means in μmole/g wet weight ± SEM. The pH assumed to be 7.2. For methods of calculation, see references Veloso and colleagues (1973) and Veech and colleagues (1979, 1994).

^ap < 0.05 between acetate-infused and saline.

^bp < 0.05 between acetate- and ethanol-infused groups, paired t-test with Bonferroni adjustment for multiple group comparisons.