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. Author manuscript; available in PMC: 2011 May 1.
Published in final edited form as: Mucosal Immunol. 2010 Jul 21;3(6):578–593. doi: 10.1038/mi.2010.41

Figure 5.

Figure 5

MyD88-dependence of development of CD11b+Gr1int cells. (a) Bone marrow cells were cultured with GM-CSF and LPS for 9 days and phenotypic analysis was performed using flow cytometry. The resulting cells from wild-type Balb/c mice were compared to those from MyD88-deficient (MyD88-/-) mice (left-hand panel), and those from wild-type C57BL/6 mice were compared to TRIF-deficient (TRIF-/-) mice (right-hand panel). (b) Estimation of fold-induction of CD11b+Gr1int cells in response to LPS in the lungs of WT, TLR4-/- and MyD88-/- mice. (c) MyD88 -/- mice received HDM ± LPS i.t. as in Figure 4a. 72 h after the final instillation, BAL fluid and lung tissue samples were obtained. Total and differential counts of cells recovered in the BAL fluid (left-hand panel) and H&E staining of lung sections (right-hand panels) were performed. Values shown are mean ± SD, *, P<0.05, **, P<0.005. The concentration of IL-5 in lung homogenates was measured by multiplex assay and is presented as mean ± SEM ***, P<0.001. The data shown are representative of two independent experiments.