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. 2010 Oct 20;5(10):e13388. doi: 10.1371/journal.pone.0013388

Figure 1. Direct comparison of Zn and Cd tolerance conferred by AtHMA4 and truncated versions in yeast.

Figure 1

AtHMA4-FL, AtHMA4-trunc and AtHMA4-C-term were expressed in zrc1 cot1 yeast mutant (A), wt yeast (BY4741) (B) or ycf1 mutant (C). Growth of yeast expressing these AtHMA4 versions were compared to vector (p426)-transformed controls and to either of two transport null mutants: Athma4(D401A) or AtHMA4(C357G). Plates contained SC minus uracil with 2% (w/v) galactose pH 5-5.5 and varying concentrations of Cd as CdSO4 or Zn as ZnSO4.