Figure 3. Effects of RACK1 overexpression on mAChR down regulation.

HEK cells were cotransfected with either M₁ (A) or M₂ (B) and either PCDNA3.1 (black bars) or RACK1 (white bars). Total receptors were measured by [³H]QNB binding following 8 hours of stimulation with 1 mM carbachol. Down regulation is expressed as percent of control cells. * indicates p<0.001.