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. 2010 Oct 20;5(10):e13517. doi: 10.1371/journal.pone.0013517

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Reiner et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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HEK cells stably expressing Flag-M₂ with either normal levels of RACK1 (black bar) or with low levels of RACK1 (white bar) expression. Total receptors were measured by [³H]QNB binding following 8 hours of stimulation with 1 mM carbachol. Down regulation is expressed as percent of receptor down regulated compared to unstimulated cells. * indicates p<0.001.