Table 2.
Characterization of immobilized p53: effect of fluorescent probes
| Probe | Relative fluorescence intensitya |
|---|---|
| Fluorescent Cy3 | |
| p53 + IgG Ab | 4.4 × 106 |
| BSA + IgG Ab | 2000 |
| Blank | 400 |
| Quantum dot 605 | |
| p53 + IgG Ab | 10,500 |
| BSA + IgG Ab | 500 |
| Blank | 250 |
| Si nanocrystal | |
| p53 + IgG Ab | 4400 |
| BSA + IgG Ab | 500 |
| Blank | 430 |
a
Relative fluorescence intensity was determined for Cy3 probe (emission = 600 nm, excitation = 530 nm); quantum dot probe (emission = 600 nm, excitation = 450 nm); Si nanocrystal probe (emission = 430 nm, excitation = 280 nm). Recorded values varied ∼20%.