Table 1.
Antibody | Antibody type | Immunizing antigen | Immunoreactivity in Western blotting | Immunoreactivity in IHC |
---|---|---|---|---|
Anti-DMP1-N-9B6.3a | Monoclonal | 37 kDa (N-terminal fragment) | Yes | Yes |
Anti-DMP1-C-8G10.3b | Monoclonal | 57 kDa (C-terminal fragment) | No | Yes |
Anti-DMP1-C-857c | Polyclonal | Oligopeptide (residues 471–485) | Yes | Yes |
Anti-BSP-10D9.2d | Monoclonal | Purified rat bone BSP | Yes | Yes |
Anti-OPNe | Monoclonal | Mouse recombinant OPN | Yes | Yes |
Anti-DSP-2G7.3f | Monoclonal | Purified rat dentin DSP | Yes | No |
Anti-DSP-2G12.3g | Monoclonal | Purified rat dentin DSP | No | Yes |
This monoclonal antibody (MAb) (Qin et al. 2006) was used to detect the NH2-terminal fragment of DMP1 by IHC and Western immunoblotting analysis.
This MAb (Baba et al. 2004b) was used to detect the COOH-terminal fragment of DMP1 by IHC analysis in this study.
This polyclonal antibody (Maciejewska et al. 2009) was used to detect the COOH-terminal fragment of DMP1 by Western immunoblotting analysis.
This MAb (Huang et al. 2008a) was used to detect BSP by IHC analysis and Western immunoblotting in this study.
This MAb (Santa Cruz) was used to detect OPN by IHC analysis and Western immunoblotting in this study.
This MAb (Qin et al. 2003a) was used to detect DSP by Western immunoblotting in this study.
This MAb (Baba et al. 2004a) was used to detect DSP by IHC analysis.
DMP1, dentin matrix protein 1; BSP, bone sialoprotein; OPN, osteopontin; DSP, dentin sialoprotein.