Table 1.

Antibodies used in this study

Antibody	Antibody type	Immunizing antigen	Immunoreactivity in Western blotting	Immunoreactivity in IHC
Anti-DMP1-N-9B6.3a	Monoclonal	37 kDa (N-terminal fragment)	Yes	Yes
Anti-DMP1-C-8G10.3b	Monoclonal	57 kDa (C-terminal fragment)	No	Yes
Anti-DMP1-C-857c	Polyclonal	Oligopeptide (residues 471–485)	Yes	Yes
Anti-BSP-10D9.2d	Monoclonal	Purified rat bone BSP	Yes	Yes
Anti-OPNe	Monoclonal	Mouse recombinant OPN	Yes	Yes
Anti-DSP-2G7.3f	Monoclonal	Purified rat dentin DSP	Yes	No
Anti-DSP-2G12.3g	Monoclonal	Purified rat dentin DSP	No	Yes

^aThis monoclonal antibody (MAb) (Qin et al. 2006) was used to detect the NH₂-terminal fragment of DMP1 by IHC and Western immunoblotting analysis.

^bThis MAb (Baba et al. 2004b) was used to detect the COOH-terminal fragment of DMP1 by IHC analysis in this study.

^cThis polyclonal antibody (Maciejewska et al. 2009) was used to detect the COOH-terminal fragment of DMP1 by Western immunoblotting analysis.

^dThis MAb (Huang et al. 2008a) was used to detect BSP by IHC analysis and Western immunoblotting in this study.

^eThis MAb (Santa Cruz) was used to detect OPN by IHC analysis and Western immunoblotting in this study.

^fThis MAb (Qin et al. 2003a) was used to detect DSP by Western immunoblotting in this study.

^gThis MAb (Baba et al. 2004a) was used to detect DSP by IHC analysis.

DMP1, dentin matrix protein 1; BSP, bone sialoprotein; OPN, osteopontin; DSP, dentin sialoprotein.