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. 2010 Oct 20;5(10):e13510. doi: 10.1371/journal.pone.0013510

Figure 4. Structural organization, phylogenetic conservation, and predicted products for RMEL1 (Hs.295012).

Figure 4

(A) Genome context of the gene. The longest cDNA (BC038566) suggests a four-exon gene and ESTs (BE386026, BU156136 and BQ217207) suggest two more exons (shaded boxes). Numbers indicate intron and exon lengths in nucleotides. (B) VISTA plot displaying the conservation of the gene in nine species. The upper and lower limits of the box representing each species correspond, respectively, to 100 and 50% of sequence identity. (C) Multiple alignment of the deduced amino acid sequences of the RMEL1 longest AUG-starting ORF of several primate species. (for all species, ORFs were deduced from putative transcribed sequences obtained by alignments of the human cDNA BC038566 against the genomic sequences). (D) Diagram of RNA secondary structures predicted by RNAz software. Rule indicates RNA length; pointed blue bars indicate segments predicted to form hairpin structures, and the numbers indicate the RNA-class probability (P), considered significant when greater than 0.5. Predicted RNA secondary structures for two of the segments (indicated by arrow heads) are shown.