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. 2010 Oct 20;5(10):e13522. doi: 10.1371/journal.pone.0013522

Figure 4. Binding profiles of the interactions of AHLs with artificial membrane systems.

Figure 4

Binding profiles of [A] 3-oxo-C14-HSL, [B] 3-oxo-C12-HSL and [C] 3-oxo-C10-HSL on titration to PC100% (•) or PC70%Cholesterol30% (Δ) di-8-ANEPPS labeled liposomes (400 µM) at 37°C (n = 5) normalised to DMSO controls. Profiles were fitted to simple hyperbolic and sigmoidal binding models (equations 1 and 2) and extra sum of squares F-Tests were used to determine the best fitting in each case (all hyperbolic). [D] Average saturation points and [E] Average dissociation constant of the best fitting models±SEM.