Figure 8. Effect of decreasing membrane dipole potential on membrane receptor conformation.

Binding profiles of the P-glycoprotein ligand Saquinavir with T-Lymphocytes (40,000 cells/ml) after pre-treatment with 160 µM 3-oxo-C12 HSL (•) or an equivalent volume of DMSO (final concentration did not exceed 0.5%) (Δ) at 37°C. Data were normalized for saquinavir additions by titrating cells with equivalent volumes of DMSO (×, dashed lines). Profiles were fitted to simple hyperbolic and sigmoidal binding models (equations 1 and 2) and F-Tests were used to determine the best fitting model (sigmoidal for 3-oxo-C₁₂ HSL and DMSO pre-treatments). In each experiment n = 3, ±SEM.