Table 2.
Polymorphisms in coding and non-coding regions of enterovirus (EV) 71 derived from viruses collected in different years.
| VP1 | 2A | 3C | 5'UTR | ||
|---|---|---|---|---|---|
| Genotype | Year | θs | θ | ||
| EV71-C2 | 1998 | 8.11% (86, 35) |
9.74% (50, 19) |
9.70% (61, 24) |
1.52% (41, 7) |
| EV71-B4 | 2000 | 10.03% (94, 49) |
10.69% (49, 28) |
9.55% (53, 18) |
1.54% (39, 12) |
| EV71-B4 | 2001 | 9.25% (101, 54) |
12.33% (67, 28) |
11.38% (76, 46) |
2.46% (66, 11) |
| EV71-B4 | 2002 | 8.74% (83, 47) |
8.37% (39, 28) |
9.34% (53, 35) |
1.84% (49, 23) |
| Average | 8.87% | 10.28% | 9.99% | 1.84% | |
| p value (vs. VP1) | NS | NS | |||
| p value (vs. UTR) | 0.014 | 0.014 | 0.014 | ||
| p value (vs. UTR)A | 0.021 | 0.041 | 0.041 | ||
| θa | |||||
| EV71-C2 | 1998 | 0.19% (7, 2) |
0.65% (12, 2) |
0.57% (13, 6) |
|
| EV71-B4 | 2000 | 0.40% (13, 7) |
0.66% (11, 4) |
0.80% (16, 6) |
|
| EV71-B4 | 2001 | 0.29% (11, 5) |
0.97% (19, 5) |
0.46% (11, 8) |
|
| EV71-B4 | 2002 | 0.21% (7, 3) |
0.54% (9, 1) |
0.49% (10, 4) |
|
| Average | 0.25% | 0.70% | 0.58% | ||
| p value (vs. VP1) | 0.014 | 0.014 | |||
| θa/θs (N/S)B | |||||
| EV71-C2 | 1998 | 2.35% (0.08, 0.06) |
6.69% (0.24, 0.11) |
5.87% (0.21, 0.25) |
|
| EV71-B4 | 2000 | 3.99% (0.14, 0.14) |
6.19% (0.22, 0.14) |
8.35% (0.30, 0.33) |
|
| EV71-B4 | 2001 | 3.11% (0.11, 0.09) |
7.87% (0.28, 0.18) |
4.06% (0.14, 0.17) |
|
| EV71-B4 | 2002 | 2.43% (0.08, 0.06) |
6.41% (0.23, 0.04) |
5.25% (0.19, 0.11) |
|
| Average | 2.82% (0.10, 0.09) |
6.79% (0.24, 0.12) |
5.81% (0.21, 0.22) |
||
| P-value(versus VP1) | 0.014 (0.001, NS) |
0.014 (0.014, 0.021) |
|||
Numbers in parentheses are total number of mutations and number of mutations without singletons, respectively.
p values are from the Mann-Whitney one-tailed test.
NS, not significant (p > 0.05).
A, Comparison of proportions of non-singleton mutations between different genomic regions.
B, Ratios of nonsynonymous (N) to synonymous (S) mutations.