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. Author manuscript; available in PMC: 2011 Oct 20.
Published in final edited form as: Bioconjug Chem. 2010 Oct 20;21(10):1820–1826. doi: 10.1021/bc100169y

Figure 6.

Figure 6

Released quantity of full-stack anti-PfHRP2 immunocomplex was dependent on antigen concentration and membrane modification. Samples containing anti-PfHRP2 antibody conjugate (14 μg/ml, 15 nM), PfHRP2 antigen in plasma (0 – 100 ng/ml range), and 0.4 μg/ml anti-PfHRP2 detection antibody were captured, washed with 400 μl PBS-T, and released into 15 μl fractions from pNIPAAm-modified membrane (Mn = 9000) or unmodified Loprodyne filter membrane. Total micrograms of peroxidase-conjugated anti-PfHRP2 detection antibody, per precipitated release fraction, was determined by measurement of TMB substrate color development against a standard curve.