Figure 8.
Effect of curcumin on neutrophil recruitment in thioglycollate induced aseptic peritonitis. (A) BALB/c mice received three i.p. injections of curcumin or vehicle alone every 12 hours in a total volume of 80μl. 4 hours after the last i.p. injection of curcumin or vehicle, peritonitis was induced by i.p. injection of 500μl of 3% thioglycollate (wt/vol; Sigma). 3 hours later, intra-peritoneal cells lavaged with 5mL of cold RPMI 1640 and the number of cells was determined using the Vi-Cell XR automatic cell counter and viability analyzer (Beckman-Coulter). (B) Giemsa staining of the lavaged peritoneal cells in PBS or thioglycolate injected mice. (C) The effect of curcumin on peritoneal neutrophil recovery in aseptic peritonitis. * p≤0.05 curcumin/thioglycollate vs. all other treatments (ANOVA followed by Fisher PLSD post-hoc test).