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. Author manuscript; available in PMC: 2011 May 1.
Published in final edited form as: Nat Immunol. 2010 Sep 26;11(11):1005–1013. doi: 10.1038/ni.1941

Fig. 6. HIV-stimulated IFN induction requires IRF3, TBK1 and STING.

Fig. 6

(a) Inhibition of selected innate immune genes by siRNA transfection of Trex1−/− MEFs assessed by qRT-PCR 48 h post transfection. *, P <0.01. Student's t-test. Error bars indicate S.D. of 3 independent experiments. (b) IRF3, TBK1 and STING are required for HIV-stimulated IFN-β induction in Trex1−/− cells. WT and Trex1−/− MEFs transfected with indicated siRNAs were infected with VSV-G-pseudotyped HIV and IFN-β mRNA was measured 24 hpi by qRT-PCR. The fold increase compared to si-CTL is displayed. *, P <0.01. Student's t-test. Error bars indicate S.D. of 3 independent experiments. (c) Innate immune factors involved in HIV-stimulated IFN response do not affect HIV DNA accumulation. Cytosolic HIV DNA in Trex1−/− MEFs transfected with the indicated siRNA was measured 10 hpi by qPCR. See Supplementary Fig. 4 for a model of the innate immune pathway stimulated by HIV DNA.