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. Author manuscript; available in PMC: 2011 May 1.
Published in final edited form as: Nat Immunol. 2010 Sep 19;11(11):1047–1056. doi: 10.1038/ni.1939

Figure 3. S1P1 drives differentiation of TH1 cells.

Figure 3

(a,b) Analysis of TH1 differentiation after OT-II;Rag1−/− and S1P1-Tg;OT-II;Rag1−/− mice were fed with Ova in the drinking water for 5 days. (a) Expression of IFN-γ and IL-17 and proportions of IFN-γ+ CD4 T cells. (b) Quantitative RT-PCR analysis of T-bet and IFN-γ expression in CD4 T cells (n=3). (c) Proportions of IFN-γ+ CD4 T cells in gated donor cells from OT-II;Foxp3gfp and S1P1-Tg;OT-II;Foxp3gfp mice that were transferred to naïve wild-type mice and immunized with Ova intravenously. (d) IFN-γ expression in total CD4 and CD8 T cells from MLN of wild-type and S1P1-Tg mice, detected by intracellular staining 5 h post PMA/ionomycin stimulation (left), and quantitative RT-PCR after 24 h stimulation with anti-CD3/CD28 (right) (n=3–4). Data represent four independent experiments.