Fig. 3.

Effect of cytokines on translocation of P65-NFκB to the nuclei of HELA cells. Upper panels. On the left are shown control HELA cells that display a cytoplasmic location of P65-NFκB. Following 30 min exposure to TNF-α (10 ng/ml) there is an obvious translocation of P65-NFκB (green signal) to the nucleus (stained with Hoescht blue). Scale bar = 50 μm. Lower panels. Untreated control cells are shown on the left and the right panel shows HELA cells that were incubated with 5 ng/ml IL-1β for 30 min. The arrows indicate cells that have nuclear localization of P65-NFκB. The intense small dots are within the nuclei and may indicate the position of the nucleoli which are targets for P65-NFκB. Scale bar = 100 μm. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of the article.)