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. Author manuscript; available in PMC: 2010 Oct 21.
Published in final edited form as: J Mol Biol. 2008 Jul 16;382(2):385–401. doi: 10.1016/j.jmb.2008.07.013

Fig. 7.

Fig. 7

B6-2 is internalized by αvβ6-expressing cells. Indirect immunofluorescence confocal miocroscopy analyses show detection of cell surface-bound and internalized B6-2. αvβ6-expressing (A375Pβ6, a-h) and non-expressing (A375Ppuro, i-p) cells were incubated with B6-2 for 1hr at 4°C, free scFv was subsequently removed and the cells were incubated at 37 °C for the times indicated. Zoom boxes show the predominant plasma membrane pattern of staining at 10 minutes (d) and internalized vesicular staining at 3 h (h). B6-2 was detected using rabbit anti-mouse IgG followed by Alexa Fluor® 546-labeled goat anti-rabbit IgG (red). Cells were also counterstained with Hoechst 33245 (blue). Scale bars 20 mm.