FIG. 3.

Accumulation of sorbitol in (A) rat retinal capillary cells and (B) rat capillary endothelial cells cultured up to 72 h in DMEM media containing 50 mM glucose. Media was changed every 24 h. Sorbitol accumulation was only observed in pericytes. The increase in media sorbitol concentrations at 24 and 48 h suggests that pericyte membrane permeability had increased with prolonged culture in high glucose. Mean ± SD, n = 4. *P < 0.05 versus untreated cells; **P < 0.05 versus AL-1576-treated cells.