Figure 1.

New editing events in the human BLCAP transcript. (a) Schematic representation of the BLCAP pre-mRNA. Exon 1 encoding the 5′UTR is represented in light blue with gray bars followed by the intron that divides the 5′UTR in 2. Exon 2 encodes the remaining 5′UTR (light blue with gray bars), the coding sequence (red rectangle) and the 3′UTR (light blue). Red dots indicate the editing sites in BLCAP mRNA (5′UTR and coding sequences). Red lines indicate the editing sites identified within the intron. (b) Sequences of BLCAP pre-mRNA. Adenosines in red indicate the edited sites in the intron (small letters) and exon 2 (capital letters). The underlined sequence corresponds to the coding region. The intron was found to be edited at 11 positions referred to as I1 to I11 from 5′ to 3′. The 5′UTR was edited at 3 positions referred to as: 5a, 5b and 5c. In the coding sequence, the edited positions are named according to the amino acid change they produce: Y/C, Q/R and K/R. Underneath, the sequences are shown the chromatograms of single clones after sequencing analysis of human BLCAP pre-mRNA in normal bladder tissue. As these sequences are cDNAs, the inosines are read as guanosines and the uridines as thymidines. The percentage of the editing events identified in the bladder tissue at different sites is shown in Table 3.