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. 2010 Oct 18;191(2):313–329. doi: 10.1083/jcb.201003090

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© 2010 Malureanu et al.

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Figure 7. — Cdc20 hypomorphism specifically prolongs metaphase. (A) Analysis of mitosis timing in Cdc20^+/+ and Cdc20^−/H MEFs in the presence or absence of CHX (n = 10 to 15 cells/group). Error bars represent SEM. *, P < 0.0005 versus Cdc20^+/+ (by Mann–Whitney test). P, prophase; PM, prometaphase; M, metaphase; A, anaphase. (B) Analysis of mitotic checkpoint activity of Cdc20^+/+ (n = 64 cells) and Cdc20^−/H MEFs (n = 68 cells) challenged with nocodazole (Noc) or taxol. Error bars represent SD. (C) Images of Cdc20^+/+ MEFs in which Mad2 levels were normal or depleted by RNA interference. Prometaphases stained for Cdc20, centromeres, and DNA are shown. (D) Western blot showing that Mad2 was efficiently depleted. (E) Images of wild-type and BubR1^−/− prometaphases stained for Cdc20, centromeres, and DNA. Bars, 10 µm.