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. 2010 Oct 18;191(2):259–267. doi: 10.1083/jcb.201004150

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© 2010 Uehara and Goshima

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 4. — Physiological importance of de novo interzonal MT generation in anaphase. (A) Immunostaining of MT (green), γ-tubulin (red), and chromosomes (blue) in control, augmin-depleted, or HURP-depleted cells in metaphase (meta) or anaphase (ana). Bar, 5 µm. (B) MT intensities in the metaphase spindle or interzone in anaphase. Means ± SD of three independent experiments are shown (>18 cells were analyzed for each treatment). Depletion of hDgt6 and HURP resulted in significant reduction in MT amounts in metaphase (*, P < 0.01), whereas only the former caused significant reduction in anaphase interzonal MTs (*, P < 0.0003 for hDgt6-depleted anaphase cells; P > 0.09 for HURP-depleted anaphase cells). (C) A model for the central spindle formation process. In anaphase, MTs that are formed in preanaphase (a; orange), those constantly nucleated from centrosomes (b; green), and those nucleated in the interzone near the chromosomes in an HURP-dependent manner (c; blue) are available as MT sources. (d; red) These MTs are used as the templates for MT amplification mediated by augmin and γ-TuRC. (e; purple) All of the interzonal MTs formed by processes a–d are stabilized and bundled by antiparallel MT-bundling proteins, resulting in robust central spindle formation.