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. 2010 Oct 18;191(2):281–290. doi: 10.1083/jcb.201003038

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© 2010 Jelluma et al.

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Figure 3. — Sustained Mps1 activity at kinetochores does not affect microtubule attachment or chromosome biorientation. (A) Time spent in prometaphase and metaphase of U2OS cells transfected with indicated plasmids and treated as indicated. Each horizontal bar represents a single cell. (B) Stills of Video 1 showing a LAP-Mis12-Mps1-WT–expressing U2OS cell after Mps1-IN-1 addition during metaphase. Graph shows distribution of time after Mps1-IN-1 addition for cells to initiate anaphase. Average time is 12.4 min ± 3.0 min (SD), n = 45 cells. (C) Distribution of interkinetochore distances in U2OS cells transfected and treated as indicated. For LAP-Mis12-Mps1–expressing cells, only the cells showing clear Mad2 staining on kinetochores were analyzed. (D) Cold-stable microtubules in U2OS cells expressing the indicated constructs. Examples are shown for metaphases that show no, low/medium, or high Mad2 at kinetochores. Averages of absolute total spindle intensities per cell are indicated (±SD).