Native CYP3A4, CYP3A4F215Q, and CYP3A4F215G production of raloxifene metabolites. The relative amounts of metabolites were estimated with an internal standard and concentration is expressed in arbitrary units (AU). Mutations of Phe215 reduced raloxifene dehydrogenation, demonstrated by the decreased production of GS-RA, the GSH adduct of the dehydrogenated product. (*) Indicates a significant difference (p < 0.05, n = 5) compared to native CYP3A4.