Table 1.

Residue changes at 4 positions in Mab40-L11 Vh domain

DNA clones	H33*	H50*	H96*	H97*	Expression**	Binding**
mAb40-L11	Tyr	Tyr	Phe	Thr	Yes	Yes
110		Glu	Thr	Asn	ND	ND
111	Arg	Pro			ND	ND
112	Asn	Met	Asn	Ser	ND	ND
113	Ser	Leu	Met	Ser	ND	ND
114	Ala	Arg	Leu	Ala	ND	ND
117	Ile	Lys	Thr	Ser	ND	ND
118	Pro	Trp	Ser	Asp	ND	ND
119	Pro	Ser	Leu	Arg	No	No
121	Asn	Gln	Lys	Gly	Yes	No
122	Ala	Ile	Gly	Cys	No	No
123	Cys	Met	Asp	Pro	No	No
124	Ser	Asn	Ile		Yes	No
125	Leu				Yes	No
126	Ile	Leu	Met	Gln	Yes	No
127	Lys	Thr	Arg	Ala	No	No
128	Glu	Trp	Leu	Phe	Yes (low)	No
129	Thr	Leu	Gly	Tyr	Yes	No
130	Asn	Val	Asp	Val	Yes	No
132	Leu	Leu	Ser	Cys	Yes	No
Total***	11/18	13/18	10/17	13/16	8/12	0/12

^*Residue number in heavy chain CDR. The residue names are not shown if they are not changed relative to parental clone Mab40-L11.

^**ND, Experiment was not done.

^***mAb40-L11 is not counted. For expression and binding, clones marked ND are not counted.