Table 2.

Sequences and binding activity of selected clones

Clones	H33/CDR1	H50/CDR2	H96/CDR3	H97/CDR3	sAg to mAb	RBA	sAb to mAg	IC50
Mab40-L11	Tyr	Tyr	Phe	Thr	Yes	0.1308	Yes	8.950e-010
12	Asp		Asp	Tyr	Yes	0.1358	No	N/A
13/22			Pro	Ala	Yes	0.1464	Yes	2.005e-010
21	Cys				Yes	0.1529	Yes	8.526e-008
32	Lys				Yes	0.2082	Yes	1.336e-009

The cDNA sequences of the Vh domains from 4 clones were analyzed by alignment with the parental sequence. Amino acid changes in the mutated positions were identified and listed in Table 2. An empty box indicates no changes from the parental sequence. Clones 13 and 22 show same amino acid changes and are listed together in one row. sAg to mAb: Soluble antigen binding to membrane-displayed antibody; RBA, Relative binding ability; sAb to mAg, Soluble antibody binding to membrane-expressed antigen; N/A, not applicable.