Figure 1.

EphA2 is differentially expressed in the CD31⁺ vascular vessels of human lymph nodes. 8 µm thick cryosections from human lymph node biopsies of either control subjects (reactive lymph node) (left) or CLL patients (right) were immunostained with a primary polyclonal antibody for human EphA2 followed by AlexaFluor-546 conjugated specie-specific secondary antibody (shown in red) and an AlexaFluor-488 conjugated anti human CD31 (shown in green). representative experiments are shown. Fluorescence images were acquired with a confocal microscope (Leica TCS-SP2 AOBS; objective: 20x multi-immersion, 1.20 NA).