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. 2010 Jul-Sep;4(3):409–418. doi: 10.4161/cam.4.3.11682

Figure 3.

Figure 3

(A) SFCMs were collected from control and ATRA treated MDA-MB-231 cells, grown in presence and in absence of 1,000 ng/ml EGF for 8 h. Gelatin zymography was performed using sepharose 4B bead. Lane C denotes control MDA-MB-231 cells grown in absence of ATRA and EGF. Lane1 shows pro-MMP-9 activity of MDA-MB-231 cells grown in absence of ATRA, but in presence of EGF. Lane 2 represents pro-MMP-9 activity of ATRA treated MDA-MB-231 cells, grown in presence of EGF. (B) Total protein was extracted from both control (lane −ATRA) and ATRA treated (lane +ATRA) MDA-MB-231 cells. 100 µg of protein from each extract was subjected to western transfer on nitrocellulose membrane. Membrane was developed using anti-EGFR antibody, keeping actin as internal control.