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(A) Gelatin zymography was performed using the SFCM, collected from MDA-MB-231 cells grown in absence (lane C) and in presence of ERK (lane 1), PI-3K (lane 2), MEK (lane 3), p38 (lane 4) and both ERK & PI-3K (lane 5) inhibitors. (B) Western blots were performed in control (lane −ATRA) and ATRA treated (lane +ATRA) MDA-MB-231 cells as before. Membranes were developed with anti-ERK, anti-p-ERK, anti-PI-3K and anti-p-PI-3K antibodies. Actin was used to confirm equal loading.
