Figure 7.

(A) MDA-MB-231 cells were grown without (lane −ATRA) or with (lane +ATRA) ATRA for 48 h and total protein were extracted. Western blot was performed as before using anti-RAR and anti-e-cadherin antibodies. Actin was used as internal control. (B) ELISA of RAR and CRABP was performed as before in MDA-MB-231 cells, grown in presence (Treated) and in absence (Control) of ATRA for 48 h. (C) Immunocytochemical localization of CRABP was performed in MDA-MB-231 cells grown without (Control) or with (ATRA treated) 20 µM ATRA for 2, 3 and 4 h.