Figure 3.

Lipopolysaccharide (LPS) and tobacco smoke (TS) exposure results in an increase in cytotoxicity and oedema. Sprague-Dawley rats were exposed to TS or air for 30 min twice a day for 2 days. On the morning of day 3 rats were exposed to saline of LPS (0.3 mg·mL⁻¹) for 30 min followed by TS or air 5 h later. Twenty-four hours after LPS exposure the levels of (A) LDH activity and (B) total protein in the BAL were assessed. Data are expressed as mean and SEM of n= 8 per group. **P < 0.01, ***P < 0.001 compared with air/saline controls; $P < 0.05 compared with air/LPS comtrols). BAL, broncho alveolar lavage.