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. 2010 Aug;160(8):2028–2044. doi: 10.1111/j.1476-5381.2010.00859.x

Figure 2.

Figure 2

Concentration dependent effect of NS5806 on KV4.3/KChIP2 in CHO-K1 cells. KV4.3 and KChIP2 were transiently expressed in CHO-K1 cells and measured in presence of 0 to 100 µM NS5806 as indicated in the figure. (A) Representative current recordings at different NS5806 concentrations. (B) Peak current amplitudes normalized to current amplitudes in absence of drug and plotted as function of NS5806 concentration. A sigmoidal dose-response curve was fitted to the data in the range from 0–30 µM and an EC50 of 5.3 ± 1.5 µM with and a Hill slope of 1.1 ± 0.05 and minimum and maximum values of 1.04 ± 0.02 and 1.6 ± 0.06 respectively. (C) Effect of different NS5806 concentrations on Kv4.3/KChIP2 decay. The time-constants, τ, were plotted as function of NS5806 concentration and revealed an EC50 of 25.4 ± 1.1 µM. The Hill slope was 1.08 ± 0.06 with minimum and maximum values of 7.0 ± 0.1 ms and 20.0 ± 0.5 ms respectively (n= 7–12). *P < 0.05, **P < 0.01, ***P < 0.001; significantly different in the absence and presence of NS5806. CHO-K1, Chinese hamster ovary cells; KChIP2, K channel interacting protein 2.