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. 2010 Aug;160(8):2055–2068. doi: 10.1111/j.1476-5381.2010.00860.x

Figure 1.

Figure 1

NRK-E52 and LLC-PK1 differ in response to aminoglycosides and in gap junction protein expression and function. (A and B) G418 and hygromycin induced cell injury in NRK-E52 and LLC-PK1 cells. NRK-E52 or LLC-PK1 cells in confluent cultures in 96-well plates were exposed to various concentrations of G418 or hygromycin for 48 h and cell viability was evaluated by WST assay. Data are expressed as percentage of living cells, compared with the untreated control. *P < 0.01 versus LLC-PK1 cells treated with respective G418. (C) Microscopic analysis of dye coupled cells and immunofluorescent staining of Cx43. Lucifer Yellow (LY) was pressure-injected into a single cell (arrow). Diffusion of LY into adjacent cells was recorded. Magnification, ×320. Expression of Cx43 was evaluated by staining the cells with an anti-Cx43 antibody. Magnification, ×400.