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. 2010 Aug;160(8):2055–2068. doi: 10.1111/j.1476-5381.2010.00860.x

Figure 3.

Figure 3

The gap junction inhibitor α-GA disrupts GJIC and decreases Cx43 protein levels. (A) Effect of α-GA on GJIC. NRK-E52 cells in confluent culture were either left untreated (control) or exposed to α-GA (5 µM) for 30 min. Lucifer Yellow (LY) was pressure-injected into a single cell. The number of cells into which LY was transferred from the injected cells within 3 min was counted. Data are presented as the mean ± SD (n= 8), *P < 0.01 versus basal control. (B) α-GA on Cx43 levels. NRK-E52 cells were treated with α-GA (5 µM) for the indicated times and cell protein was extracted and subjected to Western blot analysis by using an anti-Cx43 antibody. Equal loading of protein per lane was verified by reprobing the blot with an anti-β-actin antibody. (C) Immunofluorescent staining for Cx43 shows a decrease in NRK-E52 cells exposed to α-GA (5 µM) for 24 h. Magnification, ×200.