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. 2010 Aug;160(8):2055–2068. doi: 10.1111/j.1476-5381.2010.00860.x

Figure 6.

Figure 6

Over-expression of wild-type Cx43 in LLC-PK1 renders them vulnerable to hygromycin. (A) Transient transfection of LLC-PK1 cells with wild-type Cx43 on hygromycin-elicited cytotoxicity. LLC-PK1 cells were transiently transfected with a vector encoding GFP protein (pEGFP) or Cx43 (Cx43-EGFP) and cell response to G418 was evaluated by exposing the cells to different concentrations of hygromycin for 48 h. The expression of GFP and Cx43 is shown in the micrographs above the bar graphs. (B) Permanent transfection of LLC-PK1 cells with wild-type Cx43 on hygromycin-elicited cytotoxicity. LLC-PK1 cells were transfected with pEGFP or Cx43-EGFP and clones expressing a high level of GFP and Cx43 were selected, and analyzed for the cell response to hygromycin. The expression of GFP and Cx43 is shown in the micrographs above the bar graphs. Note the linear distribution of Cx43-GFP at the regions of cell-to-cell contacts. Magnification, ×400. *P < 0.01 versus respective control.