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. 2010 Sep 9;24(11):2232–2240. doi: 10.1210/me.2010-0235

Figure 1.

Figure 1

Schematic representation of the Tag transgenes. Pit-Tag, SV 40 Tag ORF (encoding both large and small Tags and their polyA signal) was placed under control of the Pit-1 5′-regulatory sequences (15 kb) (10). The transgene was released from the carrying plasmid DNA with SalI before injection. GH-Tag, Pit-1 regulatory sequences in Pit-Tag construct were replaced with hGH-N regulatory sequences (404-bp HSI encompassing fragment of the hGH-N LCR and a 500-bp segment encompassing the hGH-N minimal promoter). The GH-Tag transgene was released from the plasmid with BssHII before injection. hGH/BAC transgene, This 123-kb human BAC encompasses the five-gene hGH cluster along with the flanking genes SCN4A, CD79b, and TCAM1. The position of each gene is indicated by a labeled rectangle; exon structure is not displayed. hGH-NP, hGH-N promoter.