Figure 6.

Acetylation regulates transcriptional activity. A, K-A PR-B displays increased transcriptional activation. HeLa cells were transfected with wt or K-A PR-B as well as the 2XPRE-Luciferase plasmid and Renilla for a control. Cells were serum starved and treated for 18 h with vehicle or R5020 (10⁻⁸ m). Luciferase activity was measured in triplicate cultures, and statistical significance was determined using a t test (±sd; *, P < 0.02). B, Acetylation-dependent gene regulation. Triplicate cultures of T47D cells stably expressing wt or K-A PR-B were starved in 5% DCC and treated with vehicle or R5020 (10⁻⁸ m) for 18 h. Levels of SGK mRNA were measured by qPCR and were normalized to β-actin mRNA. Statistical significance was determined by comparing K-A treatment groups with wt treatment groups (±sd; *, P = 0.04). Experiments were performed three times. EtOH, Ethanol; RLU, relative light units.