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. Author manuscript; available in PMC: 2010 Oct 21.
Published in final edited form as: J Biol Chem. 2007 Dec 18;283(8):5069–5080. doi: 10.1074/jbc.M708734200

Fig. 6. Glutaraldehyde cross-linking.

Fig. 6

Analysis of the CYP130 cross-linked products by native- (A, C) and SDS- (B) gel electrophoresis. A, cross-linking performed at a protein concentration of 20 µM is shown. Two soluble P450 enzymes, CYP51 from Mtb and PikC from S. venezuelae, were used as controls. B, the molecular weight of the cross-linked CYP130 products was confirmed by the SDS-gel electrophoresis. C, effect of ionic strength on stability of the CYP130 aggregates is shown. As the KCl concentration increases from 0 to 300 mM, the dimer product stabilized by glutaraldehyde cross-linking persists but the formation of tetramers and higher oligomers is suppressed.