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. 2010 Sep 29;106(5):675–686. doi: 10.1093/aob/mcq169

Fig. 6.

Fig. 6.

Immunogold localization of calreticulin in control and chilled (4 h) leaves of KW 1074 (CT) and CM 109 (CS) maize inbred lines: (A) negative control – no immunogold labelling when primary antibody was omitted; (B–E) CT line showing the KMS/BS (B, C) and BS/VP (D, E) interfaces in which calreticulin localizes mostly to ER and CW in both control (B, D) and chilled (C, E) leaves; (F–I) CS line showing KMS/BS (F) and BS/VP (G) interfaces in control leaves, in which calreticulin localizes mostly to ER and CW, and KMS/BS (H) and BS/VP (I) interfaces in chilled leaves, in which calreticulin accumulates at the neck regions of the plasmodesmata, mostly at the BS side. Abbreviations: CT, chilling-tolerant line; CS, chilling-sensitive line; KMS, Kranz mesophyll; BS, bundle sheath; VP, vascular parenchyma; CW, cell wall; ER, endoplasmic reticulum; Pd, plasmodesmata. Scale bar = 100 nm.