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. 2010 Oct 21;6(10):e1001153. doi: 10.1371/journal.ppat.1001153

Figure 1. Constructs used in this study.

Figure 1

(A) Schematic representation of YFP-fused chimeric ubiquitin ligases containing the membrane targeting (C2) and late domain binding (WW) domains of WWP1 and the indicated catalytic HECT domains. Wild type and catalytically inactive mutant versions of each HECT ligase were constructed. (B) Schematic representation of the PFV Gag-derived proteins used in this study, containing a single K396R mutation, an Lck-derived membrane targeting peptide appended to the N-terminus, and the indicated late domains.