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. 2010 Oct 21;6(10):e1001153. doi: 10.1371/journal.ppat.1001153

Figure 5. Direct fusion of ubiquitin to Lck-Gag stimulates L-domain-dependent and -independent budding.

Figure 5

(A) Schematic representation of the Lck-Gag-Ub protein with a single ubiquitin moiety fused at the carboxyl terminus. Two glycine residues were removed from the C-terminus of ubiquitin to prevent its conjugation to other proteins. (B) VLP production from 293T cells expressing the Lck-Gag-Ub protein, or a control Lck-Gag(L-) protein was assessed by western blotting. (C) VLP production from 293T cells expressing Lck-Gag-Ub and increasing amounts of GFP-Vps4 (E228Q). (D) VLP release from 293T cells expressing Lck-Gag-Ub containing the indicated mutations in C-terminally fused ubiquitin.