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. 2010 Oct 21;6(10):e1001153. doi: 10.1371/journal.ppat.1001153

Figure 8. Effect of simultaneous ESCRT-I and ALIX disruption on ubiquitin-dependent and PPxY-dependent particle release.

Figure 8

(A) Quantitative Western blot analysis of VLP production from 293T cells transfected with plasmids expressing Lck-Gag-Ub, Lck-Gag-PY or MLV Gag-HA and siRNAs targeting Tsg101, ALIX or both. Corresponding cell lysates were also probed with antibodies to PFV, HA, Tsg101 and/or ALIX, as appropriate, and as indicated. (B) Quantitation of VLP release following knockdown of Tsg101, ALIX or both. Values are plotted as the mean+SD of two or three independent experiments and represent the levels of particles released relative to those released from cells transfected with control luciferase siRNAs, which was assigned a value of 1.