Table 1. Class E VPS factors and associated proteins encoding ubiquitin binding domains.

Protein	Domain type	Kd	Methods	References
Tsg101	UEV	∼500µM	SPR, NMR, structure	[7], [34], [59], [60]
Hrs	DUIM, VHS	∼300µM, ∼1.4mM	IP, SPR, structure	[34], [35], [36], [61], [62], [63]
STAM	UIM, VHS,	∼430µM, ∼220µM	IP, NMR, Y2H	[33], [64], [65], [66], this study
Eap45	GLUE	∼300µM	IP, SPR, structure	[37], [67], [68]
Eps15	UIM	∼0.36µM	IP, SPR	[35], [36]
ALIX	Unknown		IP, Y2H	[48], this study
CIN85	SH3		IP	[69]

SPR: surface plasmon resonance; NMR: nuclear magnetic resonance; IP: immunoprecipitation or bead based ‘pull-down’ assays; Y2H: yeast 2-hybrid.