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. 2010 Oct 21;6(10):e1001159. doi: 10.1371/journal.ppat.1001159

Figure 3. Microbiological properties of recombinant BCG.

Figure 3

A) In vitro multiplication of WT BCG (blue line) and r-BCG PGL-1 (magenta line). 3-week cultures of both BCG and r-BCG PGL-1 were diluted to final OD600nm = 0.001 in 7H9 broth containing ADC and 0.05% Tween 80 and incubated at 37°C. At the indicated time points, the OD600nm was measured. Resistance to H2O2 (B) or NaNO2 (C). Exponentially growing WT BCG and r-BCG PGL-1 were diluted 1∶100 in 7H9 broth supplemented with ADC and containing various concentrations of H2O2 (0, 3, 6 or 12 mM) and NaNO2 (0, 2.5 or 5mM). For NaNO2, pH was adjusted to 5.5. After 24 h of incubation with ROI or RNI, serial dilutions were plated on 7H11 supplemented with OADC and cfus were evaluated after 3 weeks of incubation at 37°C. The indicated values are means (+/− SEM) of three independent experiments.