Figure 6. Combined Perp-deficiency and chronic UVB exposure induce immune cell infiltration in the skin.

A) Representative immunofluorescence images of myeloid cells, as determined by MPO staining (arrows), in the skin of control and K14CreER;Perp^fl/fl mice treated with UVB light for 19 weeks. B) Quantification of MPO-positive cells in UVB-treated cohorts. Graph represents the average of 3 mice, quantified in triplicate 200× fields +/− SEM. (p = 0.89; Student's unpaired t-test). C) Representative immunofluorescence images of T-cells, as assessed by CD3 staining (arrows), in the skin of control and K14CreER;Perp^fl/fl mice treated with UVB light for 19 weeks. D) Quantification of CD3-positive T-cell numbers in UVB-treated cohorts. Graph represents the average number of T-cells in the skin of 3 mice, quantified in triplicate 200× fields, +/− SEM. (* p =  0.044, Student's unpaired t-test). E) Representative images of staining for mast cells, as assessed by toluidine blue-positivity (arrows), in the skin of control and K14CreER;Perp^fl/fl mice treated with UVB light for 19 weeks. Note the increase in mast cells underlying the epidermis in the K14CreER;Perp^fl/fl mice. F) Quantification of mast cells in UVB-treated cohorts. Graph represents the average of 3 mice, quantified in triplicate 200× fields +/− SEM. (* p =  0.0092; Student's unpaired t-test).