Figure 8.
PTH attenuates the inhibitory effect of oxidative stress on β-catenin/Tcf-mediated transcription. Luciferase activity (relative light units, RLU) in UAMS-32P or OB-6 cells transfected with a Tcf-luc reporter construct after pretreatment for 1 hour with PBS (veh) or 50 nM PTH, then with saline (−), 50 ng/ml Wnt3a (50 ng/ml), 100 μM H2O2, or the combination of Wnt3a and H2O2 for 24 h. Bars represent the mean of triplicate determinations. * p<0.05 vs. respective vehicle control, ‡ <0.05 vs. vehicle alone, # p<0.05 vs. Wnt3a alone by one-way ANOVA.