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. 2010 Oct 21;5(10):e13530. doi: 10.1371/journal.pone.0013530

Figure 3. Phylogenetic tree of 16S rRNA sequences from Thiomicrospira cultivated isolates and clones collected from Lost City chimneys.

Figure 3

Sequences with 100% identity to the V6 hypervariable region of clone LC1537B-12 and contig C2148 were found to be most abundant in a carbonate chimney aged 34 yrs venting fluids with a maximum temperature of 88°C [6]. Contig C2148 is 9 kb in length, has 42%GC, and has 8.7x coverage. The tree was constructed with Tree-Puzzle 5.2 [32] from 1278 characters aligned with MUSCLE [33]. Quartet puzzling support values are shown; nodes with less than 50% support are collapsed. Genbank accession numbers for each sequence from top to bottom: AF064545, AF069959, AF013972, AF064544, L40811, AB166731, AF013971, DQ270608, ACQI01002148, DQ270607, AF013973, AF013974, AJ237758, FJ792484, AF013975, AJ404732, AY575776, AF013978, FJ792098, DQ270609, AF016046, AF329082.