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. 2010 Oct 21;5(10):e13536. doi: 10.1371/journal.pone.0013536

Figure 4. Role of CDKN1A-p21 in i-Extract induced cytotoxicity and its abrogation by selected gene targets.

Figure 4

i-Extract induced growth arrest of MCF7 cells was due to their arrest at G2 cell cycle phase (A). An increase in CDKN1A-p21 expression was seen in MCF7 cells when treated with i-Extract and Withanone. No increase in CDKN1A-p21 expression was seen in normal cells in the presence of either i-Extract or Withanone (B and C). Knockdown of each of the four indicated genes that compromised i-Extract induced cytotoxicity also reverted the i-Extract induced CDKN1A-p21 upregulation (D and E). HCT116 p21−/− cells were less sensitive to i-Extract as compared to their isogenic p21+/+ cells. Cell viability in response to the serially increasing concentrations of i-Extract (F).